Proliferative generation of mammalian auditory hair cells in culture

被引:117
作者
Malgrange, B
Belachew, S
Thiry, M
Nguyen, L
Rogister, B
Alvarez, ML
Rigo, JM
Van de Water, TR
Moonen, G
Lefebvre, PP
机构
[1] Univ Liege, Ctr Cellular & Mol Neurobiol, B-4020 Liege, Belgium
[2] Univ Liege, Dept Neurol, B-4020 Liege, Belgium
[3] Univ Liege, Lab Cell & Tissue Biol, B-4020 Liege, Belgium
[4] Inst Pathol & Genet, B-6280 Gerpinnes, Belgium
[5] Univ Miami, Dept Otolaryngol, Miami, FL 33136 USA
关键词
organ of Corti; inner ear; progenitor cells; myosin VIIA; p271(KIPI); nestin; hair cells;
D O I
10.1016/S0925-4773(01)00642-6
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hair cell (HC) and supporting cell (SC) productions are completed during early embryonic development of the mammalian cochlea. This study shows that acutely dissociated cells from the newborn rat organ of Corti, developed into so-called otospheres consisting of 98% nestin (+) cells when plated on a non-adherent substratum in the presence of either epidermal growth factor (EGF) or fibroblast growth factor (FGF2). Within cultured otospheres, nestin ( 4) cells were shown to express EGF receptor (EGFR) and FGFR2 and rapidly give rise to newly formed myosin VHA (+) HCs and p27(KIP1) (+) SCs. Myosin VIIA (+) HCs had incorporated bromode oxyuridine (BrdU) demonstrating that they were generated by a mitotic process. Ultrastructural studies confirmed that HCs had differentiated within the otosphere, as defined by the presence of both cuticular plates and stereocilia. This work raises the hypothesis that nestin (+) cells might be a source of newly generated HCs and SCs in the injured postnatal organ of Corti. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:79 / 88
页数:10
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