A general method for determining the contribution of split pathways in metabolite production in the yeast Saccharomyces cerevisiae

被引:10
作者
Woldman, Y [1 ]
Appling, DR [1 ]
机构
[1] Univ Texas, Inst Cellular & Mol Biol, Dept Chem & Biochem, Austin, TX 78712 USA
关键词
D O I
10.1006/mben.2001.0221
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We describe a simple method using C-13 labeling and NMR spectroscopy to determine the flux contributions of alternative pathways in Saccharomyces cerevisiae that produce the same metabolite with identical labeling patterns. Cells were incubated with a C-13-labeled precursor for one of the branches, and the absolute enrichment of the product and its metabolic precursor(s) was quantified. The ratio of the absolute enrichment of the product to that of its precursor reflects the contribution of the pathway. The method was applied to the biosynthesis of glycine in yeast, which can occur from threonine via threonine aldolase or from serine via serine hydroxymethyltransferase. [2-C-13]Aspartate and [2-C-13]serine were used as labeled precursors for the threonine aldolase and serine hydroxymethyltransferase pathways, respectively. The data show that in cells possessing both pathways, the serine hydroxymethyltransferase pathway contributes 65-75% of the total glycine production. In comparison with other approaches, this method provides an inexpensive, flexible alternative to determining the flux contributions of split pathways under controlled conditions and should have wide applicability in the metabolic engineering of microorganisms. (C) 2002 Elsevier Science (USA).
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页码:170 / 181
页数:12
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