Development of a novel pomegranate standard and new method for the quantitative measurement of pomegranate polyphenols

被引：37

作者：

Martin, Keith R. ^{[1
]}

Krueger, Christian G. ^{[2
]}

Rodriquez, Gerardo ^{[3
]}

Dreher, Mark ^{[4
]}

Reed, Jess D. ^{[2
]}

机构：

[1] Arizona State Univ, Dept Nutr, Mesa, AZ 85212 USA

[2] Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA

[3] Natl Univ, Dept Chem, Heredia, Costa Rica

[4] POM Wonderful LLC, Los Angeles, CA 90064 USA

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2009年 / 89卷 / 01期

关键词：

pomegranate; Folin-Ciocalteu; hydrolyzable tannin; ellagitannin; polyphenol; gallic acid; MASS-SPECTROMETRY; TANNINS; ELLAGITANNIN; BARK;

D O I：

10.1002/jsfa.3423

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

BACKGROUND: The Folin-Ciocalteu method is routinely used to calculate total polyphenol concentrations (TPP) in food products. However, the use of inappropriate standards such as gallic acid (GA) may give inaccurate estimates of TPP content. A method for the production of a pomegranate (Punica granatum L. 'Wonderful') standard was developed and validated to avoid this problem. The PIPS was produced by solid phase (C18) isolation of polyphenols from POMx powder (a nutritional supplement) and used for TPP analysis. RESULTS: TPP content of POMx was >90% using PIPS, whereas the GA standard underestimated TPP by up to 30%. Compositional analysis confirmed a balance of about 9% non-polyphenolic compounds. Mass spectrometry analysis corroborated polyphenol composition and confirmed pomegranate-specific peaks, i.e., punicalagin and punicalin. CONCLUSION: Results support the use of pomegranate-specific standards to accurately determine TPIP. () 2008 Society of Chemical industry

引用

页码：157 / 162

页数：6

共 18 条

[1] Anthocyanin- and hydrolyzable tannin-rich pomegranate fruit extract modulates MAPK and NF-κB pathways and inhibits skin tumorigenesis in CD-1 mice [J].