Selective regulation of integrin-cytoskeleton interactions by the tyrosine kinase Src

被引:227
作者
Felsenfeld, DP
Schwartzberg, PL
Venegas, A
Tse, R
Sheetz, MP [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
[2] NHGRI, Genet Dis Res Branch, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1038/12021
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cell motility on extracellular-matrix (ECM) substrates depends on the regulated generation of force against the substrate through adhesion receptors known as integrins, Here we show that integrin-mediated traction forces can be selectively modulated by the tyrosine kinase Src, In Src-deficient fibroblasts, cell spreading on the ECM component vitronectin is inhibited, while the strengthening of linkages between integrin vitronectin receptors and the force-generating cytoskeleton in response to substrate rigidity is dramatically increased. In contrast, Src deficiency has no detectable effects on fibronectin-receptor function. Finally, truncated Src (lacking the kinase domain) co-localizes to focal-adhesion sites with alpha(v) but not with beta(1) integrins, These data are consistent with a selective, functional interaction between Src and the vitronectin receptor that acts at the integrin-cytoskeleton interface to regulate cell spreading and migration.
引用
收藏
页码:200 / 206
页数:7
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