Rapid secretion of interleukin-1β by microvesicle shedding

被引：729

作者：

MacKenzie, A

Wilson, HL

Kiss-Toth, E

Dower, SK

North, RA

Surprenant, A ^{[1
]}

机构：

[1] Univ Sheffield, Inst Mol Physiol, Sheffield S10 2TN, S Yorkshire, England

[2] Univ Sheffield, Sect Funct Genom, Sheffield S10 2TN, S Yorkshire, England

来源：

IMMUNITY | 2001年 / 15卷 / 05期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S1074-7613(01)00229-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The proinflammatory cytokine interleukin-1 beta (IL-1 beta) is a secreted protein that lacks a signal peptide and does not follow currently known pathways of secretion. Its efficient release from activated immune cells requires a secondary stimulus such as extracellular ATP acting on P2X(7) receptors. We show that human THP-1 monocytes shed microvesicles from their plasma membrane within 2-5 s of activation of P2X(7) receptors. Two minutes after such stimulation, the released microvesicles contained bioactive IL-1 beta, which only later appeared in the vesicle-free supernatant. We conclude that microvesicle shedding is a major secretory pathway for rapid IL-1 beta release from activated monocytes and may represent a more general mechanism for secretion of similar leaderless secretory proteins.

引用

页码：825 / 835

页数：11

共 52 条

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