IMMUNOMODULATORY ACTIVITY OF THE WATER EXTRACT OF Thymus vulgaris, Thymus daenensis, AND Zataria multiflora ON DENDRITIC CELLS AND T CELLS TESPONSES

Thymus vulgaris (thyme), Thymus daenensis, and Zataria multiflora are medicinal plants being used widely for infections and inflammatory diseases in folk medicine. In this study, the effects of the water extract of these plants on the activation of dendritic cells (DCs) and T cells was investigated. Both T. vulgaris and Z. multiflora decreased the proliferation of mitogen-stimulated lymphocytes, whereas T. daenensis induced cell proliferation in a dose-dependent manner (p < 0.001). All the three plants increased the CD40 expression on DCs (p < 0.04). The extent of allogenic Tcell proliferation in the presence of T. vulgaris and Z. multiflora extracts was significantly decreased (p < 0.02). The effect of the extracts on secretion of IFN-gamma and IL-4 cytokines showed that none of the extracts influenced the pattern of cytokine production by T helper (Th) cells toward a Th1 or Th2 profile. In conclusion, all the extracts had the ability to activate DCs. Whereas Z. multiflora and T. vulgaris extracts showed immunoihibitory effects on allogenic Tcell proliferation, the main effect of T. daenensis was on mitogenic T cell response. These data may partly explain the mechanisms underlying the beneficial immunomodulatory effects of these extracts in infections and immune-related diseases.