Ginsenoside rg1 enhances CD4+ T-cell activities and modulates Th1/Th2 differentiation

被引:98
作者
Lee, EJ
Ko, EJ
Lee, JW
Rho, SW
Ko, SG
Shin, MK
Min, BI
Hong, MC
Kim, SY
Bae, HS
机构
[1] Kyung Hee Univ, Coll Oriental Med, Dept Physiol, Seoul 130701, South Korea
[2] Kyung Hee Univ, Dept EW Med, Grad Sch, Seoul 130701, South Korea
[3] Seoul Natl Univ Hosp, Clin Res Inst, Seoul 110799, South Korea
[4] Kyung Hee Univ, Coll Med, Dept Internal Med, Seoul 130701, South Korea
关键词
Rg1; Th1; Th2; CD4(+) T cells; IL-4; IFN-gamma;
D O I
10.1016/j.intimp.2003.12.007
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Panax ginseng is commonly used as a tonic medicine in Asian countries such as Korea, China, and Japan. It has been reported that ginsenoside Rg1 in P. ginseng increases the proportion of T helper (Th) cells among the total number of T cells and promotes IL-2 gene expression in murine splenocytes. This implies that ginsenoside Rg1 increases the immune activity of CD4(+) T cells, however, the exact mechanism remains unknown. The present study elucidated the direct effect of Rg1 on helper T-cell activities and on Th1/Th2 lineage development. The results demonstrated that ginsenoside Rg1 had no mitogenic effects on unstimulated CD4(+) T cells, but augmented CD4(+) T-cell proliferation upon activation with anti-CD3/anti-CD28 antibodies in a dose-dependent manner. Rg1 also enhanced the expression of cell surface protein CD69 on CD4(+) T cells. In Th0 condition, ginsenoside Rg1 increases the expression of IL-2 mRNA, and enhances the expression of IL-4 mRNA on CD4(+) T cells, suggesting that Rg1 prefers to induce Th2 lineage development. In addition, ginsenoside Rg1 increases IL-4 secretion in CD4(+) T cells under Th2 skewed condition, while decreasing IFN-gamma secretion of cells in Th1 polarizing condition. Thus, Rg1 enhances Th2 lineage development from the naive CD4(+) T cell both by increasing Th2 specific cytokine secretion and by repressing Th1 specific cytokine production. Therefore, these results suggest that ginsenoside Rg1 is a desirable agent for enhancing CD4(+) T-cell activity, as well as the correction of Th1-dominant pathological disorders. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:235 / 244
页数:10
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