African swine fever virus-specific cytotoxic T lymphocytes recognize the 32 kDa immediate early protein (vp32)

被引：34

作者：

Alonso, F ^{[1
]}

Dominguez, J ^{[1
]}

Vinuela, E ^{[1
]}

Revilla, Y ^{[1
]}

机构：

[1] UAM,CSIC,CTR BIOL MOL SEVERO OCHOA,MADRID 28049,SPAIN

来源：

VIRUS RESEARCH | 1997年 / 49卷 / 02期

关键词：

African swine fever; cytotoxic T lymphocytes; vaccinia recombinant;

D O I：

10.1016/S0168-1702(97)01459-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

African swine fever (ASF) virus-specific cytotoxic T lymphocyte (CTL) activity has been studied in a model in which SLA inbred minipigs were experimentally infected with an attenuated isolate of the virus. The CTL assays were performed using alveolar macrophages as target cells. The specific lysis is mediated by purified CD8(+) lymphocytes but not by CD4(+) cells and can be blocked by incubation with anti-SLA class I monoclonal antibodies. The purified CD8(+) population produced high levels of interferon-gamma after ASF virus stimulation. In an attempt to define the Viral proteins recognized by CTL, target cells infected with a recombinant vaccinia virus (VV) expressing the ASF virus p32, an immediate early protein during ASF virus replication, were recognized and lysed by CTL. This assay may be useful for VV recombinant screening in order to identify other potential target ASF virus proteins. (C) 1997 Elsevier Science B.V.

引用

页码：123 / 130

页数：8

共 22 条

[1] CHARACTERIZATION OF P-30, A HIGHLY ANTIGENIC MEMBRANE AND SECRETED PROTEIN OF AFRICAN SWINE FEVER VIRUS [J].