Hantavirus Gn and Gc Glycoproteins Self-Assemble into Virus-Like Particles

被引：37

作者：

Acuna, Rodrigo ^{[1
]}

Cifuentes-Munoz, Nicolas ^{[1
]}

Marquez, Chantal L. ^{[1
]}

Bulling, Manuela ^{[1
]}

Klingstrom, Jonas ^{[2
,3
]}

Mancini, Roberta ^{[4
]}

Lozach, Pierre-Yves ^{[5
]}

Tischler, Nicole D. ^{[1
,6
]}

机构：

[1] Fdn Ciencia & Vida, Mol Virol Lab, Santiago, Chile

[2] Karolinska Univ Hosp, Karolinska Inst, Dept Med, Ctr Infect Med, Stockholm, Sweden

[3] Swedish Inst Communicable Dis Control, Dept Preparedness, Solna, Sweden

[4] ETH, Inst Biochem, Zurich, Switzerland

[5] Univ Quebec, INRS Inst Armand Frappier, Quebec City, PQ, Canada

[6] Univ Andres Bello, Fac Ciencias Biol, Santiago, Chile

来源：

JOURNAL OF VIROLOGY | 2014年 / 88卷 / 04期

关键词：

KOREAN HEMORRHAGIC-FEVER; SIN-NOMBRE-VIRUS; HANTAAN-VIRUS; CYTOPLASMIC TAIL; UUKUNIEMI-VIRUS; DNA VACCINE; NUCLEOCAPSID PROTEIN; ELECTRON-MICROSCOPY; PULMONARY SYNDROME; NONHUMAN-PRIMATES;

D O I：

10.1128/JVI.03118-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

引用

收藏

页码：2344 / 2348

页数：5

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