Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI

被引:55
作者
Bartels, Benjamin [1 ]
Svatos, Aleas [1 ]
机构
[1] Max Planck Inst Chem Ecol, Res Grp Mass Spectrometry Prote, Jena, Germany
来源
FRONTIERS IN PLANT SCIENCE | 2015年 / 6卷
关键词
ambient; ionization; mass spectrometry; laser ablation; electrospray; SINGLE-CELL LEVEL; ELECTROSPRAY-IONIZATION; ATMOSPHERIC-PRESSURE; ARABIDOPSIS-THALIANA; METABOLITES; DESORPTION; TISSUE; PROTEINS; MALDI; RESOLUTION;
D O I
10.3389/fpls.2015.00471
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
This short review aims to summarize the current developments and applications of mass spectrometry-based methods for in situ profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization and UV-laser desorption/ionization methods are reviewed. The underlying mechanisms of the ionization techniques namely, laser ablation of biological samples and electrospray ionization as well as variations of the LAESI ion source for specific targets of interest are described.
引用
收藏
页码:1 / 7
页数:7
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