The MIC1 microneme protein of Toxoplasma gondii contains a duplicated receptor-like domain and binds to host cell surface

被引：92

作者：

Fourmaux, MN

Achbarou, A

MercereauPuijalon, O

Biderre, C

Briche, I

Loyens, A

OdbergFerragut, C

Camus, D

Dubremetz, JF

机构：

[1] INSERM,U42,F-59651 VILLENEUVE DASCQ,FRANCE

[2] INST PASTEUR,UNITE IMMUNOL MOL PARASITES,F-75015 PARIS,FRANCE

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1996年 / 83卷 / 02期

关键词：

Toxoplasma gondii; micronemes; adhesion; invasion; duplication;

D O I：

10.1016/S0166-6851(96)02773-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA encoding the Toxaplasma gondii microneme protein MIC1 and the corresponding gene have been cloned and sequenced, The MIC1 gene contains three introns. The cDNA encodes a 456 amino acid (aa) sequence, with a typical signal sequence and no other trans-membrane domain. The protein contains a tandemly duplicated domain with conservation of cysteines and presents distant homology with the Plasmodium sp, microneme protein TRAP-SSP2. The MIC1 protein from tachyzoite lysates and a PMAL recombinant expressing the N-terminal duplicated domain of the protein bound to the surface of putative host cells, suggesting a possible involvement of MIC1 in host cell binding/recognition. Copyright (C) 1996 Elsevier Science B.V.

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页码：201 / 210

页数：10

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