Molecular determinants for subcellular localization of PSD-95 with an interacting K+ channel

被引：106

作者：

Arnold, DB

Clapham, DE ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Boston, MA 02115 USA

[2] Childrens Hosp, Boston, MA 02115 USA

来源：

NEURON | 1999年 / 23卷 / 01期

关键词：

D O I：

10.1016/S0896-6273(00)80761-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

ion channels and PSD-95 are colocalized in specific neuronal subcellular locations by an unknown mechanism. To investigate mechanisms of localization, we used biolistic techniques to express GFP-tagged PSD-95 (PSD-95:GFP) and the K+-selective channel Kv1.4 in slices of rat cortex. In pyramidal cells, PSD-95:GFP required a single PDZ domain and a region including the SH3 domain for localization to postsynaptic sites. When transfected alone, PSD-95:GFP was present in dendrites but absent from axons. When cotransfected with Kv1.4, PSD-95:GFP appeared in both axons and dendrites, while Kv1.4 was restricted to axons. When domains that mediate the interaction of Kv1.4 and PSD-95 were disrupted, Kv1.4 localized nonspecifically. Our results provide evidence that Kv1.4 itself may determine its subcellular location, while an associated MAGUK protein is a necessary but not sufficient cofactor.

引用

收藏

页码：149 / 157

页数：9

相关论文

共 40 条

[1] A STRATEGY FOR THE ANALYSIS OF GENE-EXPRESSION DURING NEURAL DEVELOPMENT [J].

ARNOLD, D ;

FENG, L ;

KIM, J ;

HEINTZ, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (21) :9970-9974

[2] A calcium responsive element that regulates expression of two calcium binding proteins in Purkinje cells [J].

Arnold, DB ;

Heintz, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (16) :8842-8847

[3] Comparison of hippocampal dendritic spines in culture and in brain [J].

Boyer, C ;

Schikorski, T ;

Stevens, CF .

JOURNAL OF NEUROSCIENCE, 1998, 18 (14) :5294-5300

[4] Requirement for the PDZ domain protein, INAD, for localization of the TRP store-operated channel to a signaling complex [J].

Chevesich, J ;

Kreuz, AJ ;

Montell, C .

NEURON, 1997, 18 (01) :95-105

[5] THE RAT-BRAIN POSTSYNAPTIC DENSITY FRACTION CONTAINS A HOMOLOG OF THE DROSOPHILA DISKS-LARGE TUMOR SUPPRESSOR PROTEIN [J].

CHO, KO ;

HUNT, CA ;

KENNEDY, MB .

NEURON, 1992, 9 (05) :929-942

[6] Binding of the inward rectifier K+ channel Kir 2.3 to PSD-95 is regulated by protein kinase A phosphorylation [J].

Cohen, NA ;

Brenman, JE ;

Snyder, SH ;

Bredt, DS .

NEURON, 1996, 17 (04) :759-767

[7]

Cooper EC, 1998, J NEUROSCI, V18, P965

[8] Improved green fluorescent protein by molecular evolution using DNA shuffling [J].

Crameri, A ;

Whitehorn, EA ;

Tate, E ;

Stemmer, WPC .

NATURE BIOTECHNOLOGY, 1996, 14 (03) :315-319

[9] GRIP: A synaptic PDZ domain-containing protein that interacts with AMPA receptors [J].

Dong, HL ;

OBrien, RJ ;

Fung, ET ;

Lanahan, AA ;

Worley, PF ;

Huganir, RL .

NATURE, 1997, 386 (6622) :279-284

[10] Crystal structures of a complexed and peptide-free membrane protein-binding domain: Molecular basis of peptide recognition by PDZ [J].

Doyle, DA ;

Lee, A ;

Lewis, J ;

Kim, E ;

Sheng, M ;

MacKinnon, R .

CELL, 1996, 85 (07) :1067-1076

← 1 2 3 4 →