Intranasal immunization with human papillomavirus type 16 capsomeres in the presence of non-toxic cholera toxin-based adjuvants elicits increased vaginal immunoglobulin levels

被引：27

作者：

Dell, K

Koesters, R

Linnebacher, M

Klein, C

Gissmann, L

机构：

[1] Deutsch Krebsforschungszentrum, D-69120 Heidelberg, Germany

[2] Univ Klin Heidelberg, Inst Pathol, Abt Angew Tumorbiol, D-69120 Heidelberg, Germany

[3] Univ Klin Rostock, Allgemeinchirurg Abt, D-18057 Rostock, Germany

来源：

VACCINE | 2006年 / 24卷 / 13期

关键词：

intranasal immunization; capsomeres; CTA1-D2D1; CTB;

D O I：

10.1016/j.vaccine.2005.11.060

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Prophylactic immunization against human papillomaviruses (HPVs) aims preferentially at the generation of antibodies, which are directed against the virus capsid proteins. DNA-free virus-like particles or their pentameric subunits, the capsomeres represent suitable antigens. Here we investigated if anti-HPV16 L1 specific antibodies and U-specific CTL induced by intranasal immunization with capsomeres in sera and vaginal washings of C57B16 mice can be enhanced by co-application of the non-toxic cholera toxin adjuvants CTA1-D2D1 or CTB. We found that CTA1-D2D1 elevated L1-specific serum IgG antibodies in a dose-dependent manner and both CTA1-D2D1 and CTB significantly increased L1-specific IgA antibody levels in the vaginal lumen. Furthermore, CTA1-D2D I and CTB enhanced L1-specific CTL responses. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2238 / 2247

页数：10

共 45 条

[1] The ADP-ribosylating CTA1-DD adjuvant enhances T cell-dependent and independent responses by direct action on B cells involving anti-apoptotic bcl-2-and germinal center-promoting effects [J].