Embryonic stem cells that differentiate into RPE cell precursors in vitro develop into RPE cell monolayers in vivo

被引：45

作者：

Aoki, H

Hara, A

Nakagawa, S

Motohashi, T

Hirano, M

Takahashi, Y

Kunisada, T ^{[1
]}

机构：

[1] Gifu Univ, Grad Sch Med, Dept Tissue & Organ Dev Regenerat & Adv Med Sci, Gifu 5011194, Japan

[2] Gifu Univ, Grad Sch Med, Dept Tumor Pathol, Gifu 5011194, Japan

[3] RIKEN, Ctr Dev Biol, Dept Cell & Dev Biol, Kobe, Hyogo 6500047, Japan

[4] RIKEN, Ctr Dev Biol, Lab Vertebrate Body Plan, Kobe, Hyogo 6500047, Japan

[5] RIKEN, Ctr Dev Biol, Lab Body Patterning, Kobe, Hyogo 6500047, Japan

来源：

EXPERIMENTAL EYE RESEARCH | 2006年 / 82卷 / 02期

关键词：

embryonic stem cell; eye; retina; RPE; stem cell; transplantation;

D O I：

10.1016/j.exer.2005.06.021

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

A culture system to generate eye-like structures consisting of lens, neural retina, and retinal pigmented epithelium (RPE) cells from undifferentiated embryonic stem cells has been established. Precursors of RPE cells that differentiated in the cultures were responsive to Wnt2b signaling and identified retrospectively to form secondary colonies consisting of only RPE-like cells in eye-like structures. These transplanted eye-like structures were capable of populating the developing chick eye as neuronal retina and RPE cells. The outgrowth of a single cell layer of mature RPE cells from the grafted eye-like structures confirmed the existence of precursors for RPE cells. These results suggest that the eye-like structures resulted from the normal developmental pathway responsible for generating eyes in vivo. If a functional effect of these cells can be established, such eye-like structures may be potentially used to establish therapy models for various eye diseases. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：265 / 274

页数：10

共 45 条

[1] Restoration of visual responses following transplantation of intact retinal sheets in rd mice [J].