Quantitative analysis of flagellar movement in hyperactivated and Acrosome-reacted golden hamster spermatozoa

被引:56
作者
Ishijima, S
Baba, SA
Mohri, H
Suarez, SS
机构
[1] Tokyo Inst Technol, Dept Bioengn, Grad Sch Biosci & Biotechnol, Meguro Ku, Tokyo 1528551, Japan
[2] Ochanomizu Univ, Fac Sci, Dept Biol, Tokyo 112, Japan
[3] Okazaki Natl Res Inst, Okazaki, Aichi 444, Japan
[4] Cornell Univ, Dept Anat Biomed Sci, Ithaca, NY USA
关键词
acrosome reaction; flagella; hyperactivation; sperm motility; spermatozoa;
D O I
10.1002/mrd.10017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Caudal epididymal spermatozoa of golden hamsters were incubated in capacitation medium. Their movement patterns changed as they became hyperactivated and underwent the acrosome reaction, To understand the basic mechanism by which changes in movement pattern are brought about, digital image analysis was carried out on the flagellar movements recorded with a video system. The degree of flagellar bending increased with incubation time, especially in the proximal midpiece. The hyperactivated spermatozoa had remarkably asymmetrical flagellar waves of large amplitude because either the bends in the same direction as the hook of the head (referred as the "pro-hook bend") or the bends in the opposite direction to the hook of the head (referred as the "anti-hook bend") extremely increased their curvature; whereas, the acrosome-reacted spermatozoa had relatively symmetrical flagellar waves of large amplitude because both the pro- and anti-hook bends remarkably increased their curvature. Beat frequency significantly decreased while wavelength of flagellar waves increased after hyperactivation and further after the acrosome reaction. These results suggest that both extreme pro- and anti-hook bends are essential in the acrosome-reacted spermatozoa even though beat frequency decreased markedly. Mol. Reprod. Dev. 61: 376-384, 2002 (C) 2002 Wiley-Liss, Inc.
引用
收藏
页码:376 / 384
页数:9
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