sel-7, a positive regulator of lin-12 activity, encodes a novel nuclear protein in Caenorhabditis elegans

被引:9
作者
Chen, JB
Li, XJ
Greenwald, I
机构
[1] Columbia Univ, Coll Phys & Surg, Dept Biochem & Mol Biophys, New York, NY 10032 USA
[2] Columbia Univ, Coll Phys & Surg, Integrated Program Cellular Mol & Biophys Studies, New York, NY 10032 USA
[3] Columbia Univ, Coll Phys & Surg, Howard Hughes Med Inst, New York, NY 10032 USA
关键词
D O I
10.1534/genetics.166.1.151
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Suppressor genetics in C. elegans has identified key components of the LIN-12/Notch signaling pathway. Here, we describe a genetic and molecular characterization of the suppressor gene sel-7. We show that reducing or eliminating sel-7 activity suppresses the effects of constitutive lin-12 activity, enhances the effects of partially reduced lin-12 activity, and causes a synthetic Lin-12(0) phenotype when combined with a null mutation in the sel-12 presenilin gene. These observations suggest that sel-7 is a positive regulator of lin-12 activity. We also show that SEL-7 encodes a novel nuclear protein. Through yeast two-hybrid screening, we identified an apparent interaction partner, K08E3.8, that also interacts with SEL-8, a known component of the nuclear complex that forms upon LIN-12 activation. Our data suggest potential roles for SEL-7 in the assembly or function of this nuclear complex.
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收藏
页码:151 / 160
页数:10
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