A flow analysis experiment involving enzymatic reactions and chemiluminescence

被引：9

作者：

Leite, OD

Fatibello, O

Rocha, FRP

机构：

[1] Univ Fed Sao Carlos, Dept Quim, BR-13560970 Sao Carlos, SP, Brazil

[2] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo, Brazil

来源：

QUIMICA NOVA | 2004年 / 27卷 / 02期

关键词：

chemiluminescence; flow analysis; enzymatic reactions;

D O I：

10.1590/S0100-40422004000200029

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A FLOW ANALYSIS EXPERIMENT INVOLVING ENZYMATIC REACTIONS AND CHEMILUMINESCENCE. An experiment is proposed to introduce some fundamentals of flow analysis, chemiluminescence and kinetic monitoring of enzymatic reactions in undergraduate courses. Chemiluminescence detection is performed with a simple spectrophotometer equipped with a lab-made spiral flow cell constructed from a polyethylene tube. The hydrogen peroxide produced by the glucose oxidation in the presence of glucose oxidise is continuously monitored by the reaction with luminol in alkaline media in a flow injection system. The exercise allows also the discussion of important analytical features and the comparison with different optical methods of analysis.

引用

页码：337 / 341

页数：5

共 20 条

[1] INTRODUCTION TO ENZYME-KINETICS [J].