Analysis of loss of heterozygosity in neoplastic nodules induced by diethylnitrosamine in the resistant BFF1 rat strain

被引：11

作者：

Gariboldi, M

Pascale, R

Manenti, G

De Miglio, MR

Calvisi, D

Carru, A

Dragani, TA

Feo, F ^{[1
]}

机构：

[1] Univ Sassari, Div Expt Pathol & Oncol, Dept Biomed Sci, I-07100 Sassari, Italy

[2] Ist Nazl Tumori, Dept Expt Oncol A, I-20133 Milan, Italy

来源：

CARCINOGENESIS | 1999年 / 20卷 / 07期

关键词：

D O I：

10.1093/carcin/20.7.1363

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Loss of heterozygosity (LOH) at specific chromosomal regions is a frequent event in poorly differentiated human hepatocellular carcinomas (HCCs), but rare in mouse HCCs. This behavior could depend on interspecies differences in mechanisms of hepatocarcinogenesis or in developmental stage of lesions, To verify if LOH is involved in rat hepatocarcinogenesis, we studied LOH frequency in slowly growing neoplastic nodules induced by Solt-Farber model in diethylnitrosamine-initiated BFF1 rats. We analyzed, with microsatellites, markers at 67 rat loci dispersed over all chromosomes, corresponding to regions homologous to those lost in human HCCs or containing hepatocellular susceptibility (Hcs) or resistance (Hcr) loci in rat and mouse. In agreement with previous findings with mouse HCCs, but at variance with human HCCs, no detectable LOH was found at any locus in rats, suggesting rare LOH involvement in neoplastic nodules, with low tendency to progress to full malignancy, of BFF1 rats.

引用

页码：1363 / 1368

页数：6

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