Kinetic of in vitro generation of some hemorphins: Early release of LVV-hemorphin-7, precursor of VV-hemorphin-7

被引：11

作者：

Dagouassat, N ^{[1
]}

Garreau, I ^{[1
]}

Zhao, Q ^{[1
]}

Sannier, F ^{[1
]}

Piot, JM ^{[1
]}

机构：

[1] POLE SCI & TECHNOL,LAB GENIE PROT & CELLULAIRE,F-17042 LA ROCHELLE 1,FRANCE

来源：

NEUROPEPTIDES | 1996年 / 30卷 / 01期

关键词：

OPIOID-PEPTIDES; BETA-CASOMORPHINS; GASTRIC TISSUES; HEMOGLOBIN; HYDROLYSIS; EXTRACTION; EXORPHINS; INVIVO; CHAIN; ACID;

D O I：

10.1016/S0143-4179(96)90047-5

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Bovine globin has been hydrolysed by pepsin to different degrees of hydrolysis. Analysis of the hydrolysates, by reversed-phase high-performance liquid chromatography (RP-HPLC), shows the release of LW- and W-hemorphin-7. LW-hemorphin-7 was the first generated, at a degree of hydrolysis (DH), as low as 4%. In contrast, W-hemorphin-7 was produced later. Our study clearly shows that W-hemorphin-7 is issued directly from LW-hemorphin-7, since this later completely disappeared during hydrolysis. This work allows us to suggest a possible pathway for in vivo hemorphins appearance.

引用

页码：1 / 5

页数：5

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