Purification and identification of components of γ-Oryzanol in rice bran oil

被引：276

作者：

Xu, ZM ^{[1
]}

Godber, JS ^{[1
]}

机构：

[1] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Dept Food Sci, Baton Rouge, LA 70803 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 07期

关键词：

gamma-Oryzanol; ferulate; rice bran oil;

D O I：

10.1021/jf981175j

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

High-purity gamma-oryzanol was obtained from crude rice bran oil using a normal-phase preparative scale HPLC. A reverse-phase HPLC method was used for separating the individual components of gamma-oryzanol present in rice bran oil. Ten fractions were isolated and collected using the reverse-phase HPLC method, and their structures were identified. Identification was accomplished using GC/MS with an electron impact mass spectrum after components were transformed into trimethylsilyl ether derivatives. The 10 components of gamma-oryzanol were identified as Delta(7)-stigmastenyl ferulate, stigmasteryl ferulate, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, Delta(7)-campestenyl ferulate, campesteryl ferulate, Delta(7)-sitostenyl ferulate, sitosteryl ferulate, compestanyl ferulate, and sitostanyl ferulate. Three of these, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate, were major components of gamma-oryzanol.

引用

页码：2724 / 2728

页数：5

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