Purification and identification of components of γ-Oryzanol in rice bran oil

被引:276
作者
Xu, ZM [1 ]
Godber, JS [1 ]
机构
[1] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Dept Food Sci, Baton Rouge, LA 70803 USA
关键词
gamma-Oryzanol; ferulate; rice bran oil;
D O I
10.1021/jf981175j
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
High-purity gamma-oryzanol was obtained from crude rice bran oil using a normal-phase preparative scale HPLC. A reverse-phase HPLC method was used for separating the individual components of gamma-oryzanol present in rice bran oil. Ten fractions were isolated and collected using the reverse-phase HPLC method, and their structures were identified. Identification was accomplished using GC/MS with an electron impact mass spectrum after components were transformed into trimethylsilyl ether derivatives. The 10 components of gamma-oryzanol were identified as Delta(7)-stigmastenyl ferulate, stigmasteryl ferulate, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, Delta(7)-campestenyl ferulate, campesteryl ferulate, Delta(7)-sitostenyl ferulate, sitosteryl ferulate, compestanyl ferulate, and sitostanyl ferulate. Three of these, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate, were major components of gamma-oryzanol.
引用
收藏
页码:2724 / 2728
页数:5
相关论文
共 11 条
[1]   SEPARATION OF VITAMIN-E AND GAMMA-ORYZANOLS FROM RICE BRAN BY NORMAL-PHASE CHROMATOGRAPHY [J].
DIACK, M ;
SASKA, M .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1994, 71 (11) :1211-1217
[2]   EXTRACTION AND IDENTIFICATION OF ANTIOXIDANTS IN OATS [J].
DUVE, KJ ;
WHITE, PJ .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1991, 68 (06) :365-370
[3]   ISOLATION AND CHARACTERIZATION OF INTACT STERYL FERULATES FROM SEEDS [J].
EVERSHED, RP ;
SPOONER, N ;
PRESCOTT, MC ;
GOAD, LJ .
JOURNAL OF CHROMATOGRAPHY, 1988, 440 :23-35
[4]   INTESTINAL AND FECAL STEROLS IN GERMFREE AND CONVENTIONAL RATS - BILE ACIDS AND STEROIDS 172 [J].
GUSTAFSSON, BE ;
GUSTAFSSON, JA ;
SJOVALL, J .
ACTA CHEMICA SCANDINAVICA, 1966, 20 (07) :1827-+
[5]  
Nicolsi R.J., 1993, RICE SCI TECHNOLOGY, P421
[6]   QUANTITATION OF STERYL FERULATE AND P-COUMARATE ESTERS FROM CORN AND RICE [J].
NORTON, RA .
LIPIDS, 1995, 30 (03) :269-274
[7]  
NORTON RA, 1994, CEREAL CHEM, V71, P111
[8]   IDENTIFICATION AND QUANTITATION OF GAMMA-ORYZANOL COMPONENTS AND SIMULTANEOUS ASSESSMENT OF TOCOLS IN RICE BRAN OIL [J].
ROGERS, EJ ;
RICE, SM ;
NICOLOSI, RJ ;
CARPENTER, DR ;
MCCLELLAND, CA ;
ROMANCZYK, LJ .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1993, 70 (03) :301-307
[9]  
RUKMINI C, 1991, J AM COLL NUTR, V10, P593
[10]   STUDIES ON HYPOCHOLESTEROLEMIC ACTIVITY OF RICE BRAN OIL [J].
SEETHARAMAIAH, GS ;
CHANDRASEKHARA, N .
ATHEROSCLEROSIS, 1989, 78 (2-3) :219-223