Recognition of the let-7g miRNA precursor by human Lin28B

被引：28

作者：

Ali, P. Shaik Syed ^{[1
]}

Ghoshdastider, Umesh ^{[1
,3
]}

Hoffmann, Jan ^{[2
]}

Brutschy, Bernd ^{[2
]}

Filipek, Slawomir ^{[3
]}

机构：

[1] Goethe Univ Frankfurt, Inst Biophys Chem, D-60438 Frankfurt, Germany

[2] Goethe Univ Frankfurt, Inst Phys & Theoret Chem, D-60438 Frankfurt, Germany

[3] Univ Warsaw, Fac Chem, PL-02093 Warsaw, Poland

来源：

FEBS LETTERS | 2012年 / 586卷 / 22期

关键词：

Lin28B; Let-7g; Pre-miRNA; MICRORNA BIOGENESIS; CAENORHABDITIS-ELEGANS; RNA-INTERFERENCE; BINDING PROTEIN; URIDYLATION; REGULATOR; FAMILY; DICER;

D O I：

10.1016/j.febslet.2012.09.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mammalian homologs of lin28: Lin28 and Lin28B block the post-transcriptional processing of the let-7 family of miRNAs. We report that in vitro the terminal stem-loop region of the let-7g miRNA precursor (pre-let-7g) required to bind Lin28B is restricted to 24 nucleotides (nt) including the 3' GGAG motif. Additionally, full length Lin28B is required for efficient binding to pre-let-7g and the stoichiometry of the complex is 1:1. Molecular dynamics IMD) simulations reveal the interactions of the pre-let-7g stem-loop and the GGAG motif in the stem region to the cold shock domain (CSD) and to the zinc knuckle domain (ZKD) of Lin28B, respectively. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3986 / 3990

页数：5

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