X-ray absorption linear dichroism spectroscopy (XALDS) on the Photosystem II manganese complex: Radiation damage and S-1-state K-edge spectra

被引：18

作者：

Dau, H ^{[1
]}

Dittmer, J ^{[1
]}

Iuzzolino, L ^{[1
]}

Schiller, H ^{[1
]}

Dorner, W ^{[1
]}

Heinze, I ^{[1
]}

Sole, VA ^{[1
]}

Nolting, HF ^{[1
]}

机构：

[1] EMBL HAMBURG OUTSTN,D-22603 HAMBURG,GERMANY

来源：

JOURNAL DE PHYSIQUE IV | 1997年 / 7卷 / C2期

关键词：

D O I：

10.1051/jp4/1997112

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

X-ray absorption spectroscopy (XAS) at the Mn K-edge is used to unravel the structure of the oxygen-evolving manganese complex which is embedded in the Photosystem II (PS II) protein complex; measurements on multilayers of PS II-containing membrane sheets provide angular information (XALDS). (1) By XAS and electron spin resonance (ESR) we investigated origin and characteristics of the radiation damage which is caused by prolonged exposure to intense synchrotron radiation. (2) By a derivative analysis of XALD-edge spectra, eight distinct transitions are resolvable (a to h). In contrast to the transitions c to e of the edge rise, f (6562 eV) and g (6565 eV) on top of the Mn K-edge exhibit characteristics of a formally-allowed electric dipole transition with an angle between transition dipole and membrane normal greater (f) and smaller (g) than the magic angle (= 54.7 degrees), respectively.

引用

页码：607 / 610

页数：4

共 7 条

[1] STRUCTURAL CONSEQUENCES OF AMMONIA BINDING TO THE MANGANESE CENTER OF THE PHOTOSYNTHETIC OXYGEN-EVOLVING COMPLEX - AN X-RAY-ABSORPTION SPECTROSCOPY STUDY OF ISOTROPIC AND ORIENTED PHOTOSYSTEM-II PARTICLES [J].