FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia

被引：50

作者：

Hobza, R

Lengerova, M

Cernohorska, H

Rubes, J

Vyskot, B

机构：

[1] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic

[2] Vet Res Inst, CZ-62132 Brno, Czech Republic

来源：

CHROMOSOME RESEARCH | 2004年 / 12卷 / 03期

关键词：

fluorescence in situ hybridization; laser microdissection; sex chromosomes; Silene latifolia;

D O I：

10.1023/B:CHRO.0000021929.97208.1c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present an improved FISH strategy for differentiating the sex chromosomes of the dioecious model plant, Silene latifolia. Fixed mitotic protoplasts were dropped on a polyethylene naphthalate membrane, the X or Y chromosomes were isolated using nitrogen laser beam microdissection, catapulted by laser pressure, and amplified by DOP-PCR. A modified FAST-FISH protocol based on a short hybridization time combined with a low concentration of probe was used. The success of this approach is demonstrated by the differential labeling of the X and Y chromosomes and it could represent a quick method for comparing organization of plant genomes.

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页码：245 / 250

页数：6

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