Carbohydrate and Domain Architecture of an Immature Antibody Glycoform Exhibiting Enhanced Effector Functions

被引：48

作者：

Crispin, Max ^{[1
]}

Bowden, Thomas A. ^{[1
]}

Coles, Charlotte H. ^{[1
]}

Harlos, Karl ^{[1
,2
]}

Aricescu, A. Radu ^{[1
]}

Harvey, David J. ^{[3
]}

Stuart, David I. ^{[1
]}

Jones, E. Yvonne ^{[1
]}

机构：

[1] Wellcome Trust Ctr Human Genet, Div Struct Biol, Oxford OX3 7BN, England

[2] Wellcome Trust Ctr Human Genet, Oxford Prot Prod Facil, Oxford OX3 7BN, England

[3] Univ Oxford, Dept Biochem, Oxford Glycobiol Inst, Oxford OX1 3QU, England

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2009年 / 387卷 / 05期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

antibody; glycosylation; oligomannose; antibody engineering; kifunensine; ASSISTED-LASER-DESORPTION/IONIZATION; DEPENDENT CELLULAR CYTOTOXICITY; HUMAN FC FRAGMENT; N-LINKED GLYCANS; MASS-SPECTROMETRY; CRYSTAL-STRUCTURE; HIGH-MANNOSE; COMPLEX; GLYCOSYLATION; IGG1;

D O I：

10.1016/j.jmb.2009.02.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1061 / 1066

页数：6

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