Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system

被引：14

作者：

Chang, Chun-Li ^{[1
,2
]}

Zhang, Hui ^{[3
]}

Shu, Dan ^{[3
]}

Guo, Peixuan ^{[3
]}

Savran, Cagri A. ^{[1
,2
,4
,5
]}

机构：

[1] Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA

[2] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA

[3] Univ Cincinnati, Dept Biomed Engn, Coll Engn & Coll Med, Vontz Ctr Mol Studies, Cincinnati, OH 45267 USA

[4] Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA

[5] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA

来源：

APPLIED PHYSICS LETTERS | 2008年 / 93卷 / 15期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1063/1.3000606

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We report a simple and robust magnetomechanical system for direct visual observation of the DNA packaging behavior of the bacteriophage phi29 in real time. The system comprises a micron-sized magnetic bead attached to the free end of the viral DNA, a magnet and a bright-field microscope. We show that the phi29 DNA packaging activity can be observed and dynamically analyzed at the single molecular level in bright field with a relatively simple system. With this system we also visually demonstrate the phi29 motor transporting a cargo 10 000 times the viral size. (C) American Institute of Physics. [DOI: 10.1063/1.3000606]

引用

页数：3

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