Expression and characterization of aiiA gene from Bacillus subtilis BS-1

被引：52

作者：

Pana, Jieru ^{[1
]}

Huang, Tianeei ^{[1
]}

Yao, Fan ^{[1
]}

Huang, Zhipeng ^{[1
]}

Powell, Charles A. ^{[2
]}

Qiu, Sixin ^{[1
]}

Guan, Xiong ^{[1
]}

机构：

[1] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, Fuzhou 350002, Fujian, Peoples R China

[2] Univ Florida, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA

来源：

MICROBIOLOGICAL RESEARCH | 2008年 / 163卷 / 06期

基金：

中国国家自然科学基金;

关键词：

aiiA gene; Bacillus subtilis; Bioassay; Expression; Sequence analysis;

D O I：

10.1016/j.micres.2007.12.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

AHL-lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus thuringiensis, Bacillus cereus and Bacillus anthracis, specifically hydrolyzes N-acyl-homoserine lactones (AHLs) secreted by Gram-negative bacteria and thereby attenuates the symptoms caused by plant pathogens. In this study, an aiiA gene was cloned from Bacillus subtilis BS-1 by PCR with a pair of degenerate primers. The deduced 250 amino acid sequence contained two small conserved regions, (SHLHFDH109)-S-103 and (166)TPGHTPG(173), which are characteristic of the metallo-beta-lactamase family. Homology comparison revealed that the deduced amino acid sequence had a high degree of similarity with those of the known AiiA proteins in the B. cereus group. Additionally, the aiiA gene was expressed in Escherichia coli BL21 (DE3) pLysS and the expressed AiiA protein could attenuate the soft rot symptoms caused by Erwinia carotovora var. carotovora. (c) 2007 Elsevier GmbH. All rights reserved.

引用

页码：711 / 716

页数：6

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