Search for optimal coupling reagent in multiple peptide synthesizer

被引：31

作者：

Hachmann, J ^{[1
]}

Lebl, M ^{[1
]}

机构：

[1] Illumina Inc, San Diego, CA 92121 USA

来源：

BIOPOLYMERS | 2006年 / 84卷 / 03期

关键词：

coupling reagents; model peptide sequences; reagents; plate-based multiple parallel centrifugation synthesizer; difficult sequences; reagent stability; mixed coupling reagents;

D O I：

10.1002/bip.20482

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ten different coupling reagents and their combinations were tested in parallel in the synthesis of four model peptide sequences. Significant differences were found between uronium and phosphonium salt-based reagents and carbodiimide. Diisopropylcarbodiimide was identified as an optimal reagent based on the purity of the product, stability of the reagent, and convenience of handling on plate-based multiple parallel centrifugation synthesizer. (c) 2006 Wiley periodicals, Inc.

引用

页码：340 / 347

页数：8

共 18 条

[1] Use of onium salt-based coupling reagents in peptide synthesis [J].