Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits

被引：269

作者：

Ayub, R ^{[1
]}

Guis, M ^{[1
]}

BenAmor, M ^{[1
]}

Gillot, L ^{[1
]}

Roustan, JP ^{[1
]}

Latche, A ^{[1
]}

Bouzayen, M ^{[1
]}

Pech, JC ^{[1
]}

机构：

[1] ECOLE NATL SUPER AGRON TOULOUSE, UA INRA, F-31076 TOULOUSE, FRANCE

来源：

NATURE BIOTECHNOLOGY | 1996年 / 14卷 / 07期

关键词：

ethylene; cantaloupe Charentais melon; Cucumis melo;

D O I：

10.1038/nbt0796-862

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The plant hormone ethylene plays a major role in the ripening of climacteric fruit. We have generated transgenic cantaloupe Charentais melons expressing an antisense ACC oxidase gene; ACC oxidase catalyzes the last step of ethylene biosynthesis. Ethylene production of transgenic fruit was <1% of control untransformed fruit, and the ripening process was blocked both on and off the vine. The antisense phenotype could be reversed by exogenous ethylene treatment. Analysis of antisense ACC oxidase melons indicated that the ripening process includes ethylene-dependent and ethylene-independent pathways. Because the transgenic line we generated displays extended storage life and improved quality, it has a promising potential for commercial development.

引用

页码：862 / 866

页数：5

共 32 条

[1]

An G., 1988, Plant Molecular Biology Manual, P1, DOI DOI 10.1007/978-94-009-0951-9

[2] ISOLATION OF A RIPENING AND WOUND-INDUCED CDNA FROM CUCUMIS-MELO L ENCODING A PROTEIN WITH HOMOLOGY TO THE ETHYLENE-FORMING ENZYME [J].

BALAGUE, C ;

WATSON, CF ;

TURNER, AJ ;

ROUGE, P ;

PICTON, S ;

PECH, JC ;

GRIERSON, D .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 212 (01) :27-34

[3]

BOUZAYEN M, 1993, CURR PLANT SCI BIOT, V16, P76

[4]

DEVAUX RD, 1976, ANN TECHNOL AGR, V25, P309

[5]

Doyle J. J., 1990, Focus, V12, P13, DOI DOI 10.2307/2419362

[6]

DUPILLE E, 1993, PLANTA, V190, P65, DOI 10.1007/BF00195676

[7] RAPID FLOW CYTOMETRIC ANALYSIS OF THE CELL-CYCLE IN INTACT PLANT-TISSUES [J].

GALBRAITH, DW ;

HARKINS, KR ;

MADDOX, JM ;

AYRES, NM ;

SHARMA, DP ;

FIROOZABADY, E .

SCIENCE, 1983, 220 (4601) :1049-1051

[8] REDUCED ETHYLENE SYNTHESIS BY TRANSGENIC TOMATOES EXPRESSING S-ADENOSYLMETHIONINE HYDROLASE [J].

GOOD, X ;

KELLOGG, JA ;

WAGONER, W ;

LANGHOFF, D ;

MATSUMURA, W ;

BESTWICK, RK .

PLANT MOLECULAR BIOLOGY, 1994, 26 (03) :781-790

[9] IDENTIFICATION OF A TOMATO GENE FOR THE ETHYLENE-FORMING ENZYME BY EXPRESSION IN YEAST [J].

HAMILTON, AJ ;

BOUZAYEN, M ;

GRIERSON, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7434-7437

[10] ANTISENSE GENE THAT INHIBITS SYNTHESIS OF THE HORMONE ETHYLENE IN TRANSGENIC PLANTS [J].

HAMILTON, AJ ;

LYCETT, GW ;

GRIERSON, D .

NATURE, 1990, 346 (6281) :284-287

← 1 2 3 4 →