A comparison of deconvolution methods as applied to high performance liquid chromatography-diode array detector-electrospray mass spectrometry of 2-and 3-hydroxypyridine at varying pH in the presence of severely tailing peak shapes

被引：18

作者：

Dunkerley, S ^{[1
]}

Brereton, RG ^{[1
]}

Crosby, J ^{[1
]}

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

来源：

CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS | 1999年 / 48卷 / 01期

基金：

英国工程与自然科学研究理事会;

关键词：

HPLC-DAD; HPLC-MS; chemometrics; deconvolution; factor analysis;

D O I：

10.1016/S0169-7439(99)00017-9

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

Triply coupled high performance liquid chromatography-diode array detection-electrospray mass spectrometry of a mixture of 2- and 3-hydroxypyridine were recorded at pH 5.0, 5.1 acid 5.2. Ar each pH the resolution changes with almost complete overlap at pH 5.1. A variety of chemometric methods including raw, normalised and standardised principal component analysis (PCA), derivatives, evolving factor analysis (EFA), fixed size window EFA, spectral similarity indices (SSI) and peak ratios are applied to the data for both detectors and all pH. Methods of deconvolution are also applied to both the diode array and mass spectral data. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：99 / 119

页数：21

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