A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants

被引：347

作者：

Marston, A ^{[1
]}

Kissling, J ^{[1
]}

Hostettmann, K ^{[1
]}

机构：

[1] Univ Lausanne, Inst Pharmacog & Phytochim, CH-1015 Lausanne, Switzerland

来源：

PHYTOCHEMICAL ANALYSIS | 2002年 / 13卷 / 01期

关键词：

bioautography; enzymatic assay; TLC; cholinesterase inhibitors; alkaloids; galanthamine; Galanthus nivalis;

D O I：

10.1002/pca.623

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A simple and rapid bioautographic enzyme assay on TLC plates has been developed for the screening of acetyleholinesterase and butyrylcholinesterase inhibition by plant extracts. Enzyme activity was detected by the conversion of naphthyl acetate into naphthol and the formation of the corresponding purple-coloured diazonium dye with Fast Blue B salt. Inhibitors of cholinesterases produced white spots on the dye-coloured background of the TLC plates. The alkaloids galanthamine and physostigmine, which are known inhibitors of acetylcholinesterase, were used to determine the sensitivity of the assay. Various plant extracts were tested using the bioassay. Copyright (C) 2001 John Wiley Sons, Ltd.

引用

页码：51 / 54

页数：4

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