Directed evolution of enzymes and pathways for industrial biocatalysis

被引：133

作者：

Zhao, HM ^{[1
]}

Chockalingam, K ^{[1
]}

Chen, ZL ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem Engn, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2002年 / 13卷 / 02期

关键词：

D O I：

10.1016/S0958-1669(02)00291-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Directed evolution has become a powerful tool for developing enzyme and whole cell based biocatalysts. Significant recent advances include the creation of novel enzyme functions and the development of several new efficient directed evolution methods The combination of directed evolution and rational design promises to accelerate the development of biocatalysts for applications in the pharmaceutical, chemical and food industries.

引用

页码：104 / 110

页数：7

共 66 条

[1] High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2 [J].

Abecassis, Valerie ;

Pompon, Denis ;

Truan, Gilles .

NUCLEIC ACIDS RESEARCH, 2000, 28 (20) :E88

[2] RETRACTED: Directed evolution of new catalytic activity using the α/β-barrel scaffold (Retracted article. See vol 417, pg 468, 2002) [J].

Altamirano, MM ;

Blackburn, JM ;

Aguayo, C ;

Fersht, AR .

NATURE, 2000, 403 (6770) :617-622

[3] A P450BM-3 mutant hydroxylates alkanes, cycloalkanes, arenes and heteroarenes [J].

Appel, D ;

Lutz-Wahl, S ;

Fischer, P ;

Schwaneberg, U ;

Schmid, RD .

JOURNAL OF BIOTECHNOLOGY, 2001, 88 (02) :167-171

[4] Combinatorial and computational challenges for biocatalyst design [J].

Arnold, FH .

NATURE, 2001, 409 (6817) :253-257

[5] Directed evolution of biocatalysts [J].

Arnold, FH ;

Volkov, AA .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (01) :54-59

[6] Design by directed evolution [J].

Arnold, FH .

ACCOUNTS OF CHEMICAL RESEARCH, 1998, 31 (03) :125-131

[7] Metabolic engineering and directed evolution for the production of pharmaceuticals [J].

Chartrain, M ;

Salmon, PM ;

Robinson, DK ;

Buckland, BC .

CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (02) :209-214

[8] The commercial production of chemicals using pathway engineering [J].

Chotani, G ;

Dodge, T ;

Hsu, A ;

Kumar, M ;

LaDuca, R ;

Trimbur, D ;

Weyler, W ;

Sanford, K .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1543 (02) :434-455

[9] DNA shuffling method for generating highly recombined genes and evolved enzymes [J].

Coco, WM ;

Levinson, WE ;

Crist, MJ ;

Hektor, HJ ;

Darzins, A ;

Pienkos, PT ;

Squires, CH ;

Monticello, DJ .

NATURE BIOTECHNOLOGY, 2001, 19 (04) :354-359

[10] Redesigning the substrate specificity of an enzyme: Isocitrate dehydrogenase [J].

Doyle, SA ;

Fung, SYF ;

Koshland, DE .

BIOCHEMISTRY, 2000, 39 (46) :14348-14355

← 1 2 3 4 5 6 7 →