Chemiluminescence of β-lactam antibiotics based on the luminol reaction

被引:41
作者
Kubo, H
Saitoh, M
Murase, S
Inomata, T
Yoshimura, Y
Nakazawa, H
机构
[1] Kitasato Univ, Sch Pharmaceut Sci, Tokyo 1088641, Japan
[2] Hoshi Univ, Fac Pharmaceut Sci, Tokyo 1428501, Japan
关键词
chemiluminescence; electron spin resonance; flow injection analysis; luminol; beta-lactam antibiotics;
D O I
10.1016/S0003-2670(99)00176-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The chemiluminescence of luminol with beta-lactam antibiotics was examined by flow injection analysis. It was found that chemiluminescence was directly produced by the reaction of beta-lactam antibiotics with luminol in the presence of a catalyst in an alkaline solution without hydrogen peroxide. Hexacyanoferrate(III) and hexacyanoferrate(II) were used as catalyst for the reaction. To explain the mechanism of the chemiluminescence of luminol with beta-lactam antibiotics, electron spin resonance (ESR) measurements were performed. ESR showed that hydroxy and superoxide radicals were generated from beta-lactam antibiotics in the alkaline solution by using 5,5'-dimethyl-1-pyrroline N-oxide as a spin trap agent. Detection limits (S/N=3) of aztreonam, penicillin G, cephalothin, 6-aminopenicillanic acid, 7-aminocephalosporanic acid, panipenem, latamoxef and faropenem were 100, 60, 40, 20, 4, 2, 1 and 1 ng per injection, respectively. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:89 / 94
页数:6
相关论文
共 21 条
  • [1] ALBERT HO, 1928, Z PHYS CHEM, V136, P321
  • [2] Bowie AR, 1996, J BIOLUM CHEMILUM, V11, P61, DOI 10.1002/(SICI)1099-1271(199603)11:2<61::AID-BIO406>3.0.CO
  • [3] 2-O
  • [4] BRODERSEN R, 1947, ACTA PHARMACOL TOX, V3, P345
  • [5] CHAN S, 1991, J PHARM SCI, V80, P1017
  • [6] LUMINOL AND LUCIGENIN AS DETECTORS FOR O2.-
    FAULKNER, K
    FRIDOVICH, I
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 1993, 15 (04) : 447 - 451
  • [7] CHEMILUMINESCENCE AND BIOLUMINESCENCE
    KRICKA, LJ
    [J]. ANALYTICAL CHEMISTRY, 1995, 67 (12) : R499 - R502
  • [8] Chemiluminescence flow injection analysis of reducing agents based on the luminol reaction
    Kubo, H
    Toriba, A
    [J]. ANALYTICA CHIMICA ACTA, 1997, 353 (2-3) : 345 - 349
  • [9] Kubo H, 1998, BUNSEKI KAGAKU, V47, P599
  • [10] FLOW-INJECTION ASSAYS WITH CHEMILUMINESCENCE AND BIOLUMINESCENCE DETECTION - A REVIEW
    LEWIS, SW
    PRICE, D
    WORSFOLD, PJ
    [J]. JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1993, 8 (04): : 183 - 199