Supplement of L-Arg improves protective immunity during early-stage Plasmodium yoelii 17XL infection

被引：18

作者：

Zhu, X. ^{[1
,2
]}

Pan, Y. ^{[1
]}

Li, Y. ^{[1
]}

Cui, L. ^{[3
]}

Cao, Y. ^{[1
]}

机构：

[1] China Med Univ, Coll Basic Med Sci, Dept Immunol, Shenyang 110001, Liaoning, Peoples R China

[2] China Med Univ, Inst Pathol & Pathophysiol, Shenyang 110001, Liaoning, Peoples R China

[3] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA

来源：

PARASITE IMMUNOLOGY | 2012年 / 34卷 / 8-9期

基金：

中国国家自然科学基金;

关键词：

activation; arginine; dendritic cell; immune response; Plasmodium yoelii; rodent malaria;

D O I：

10.1111/j.1365-3024.2012.01374.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

L-arginine (L-Arg), the precursor of nitric oxide (NO), plays multiple important roles in nutrient metabolism and immune regulation. L-Arg supplement serves as a potential adjunctive therapy for severe malaria, because it improves NO bioavailability and reverses endothelial dysfunction in severe malaria patients. In this study, we investigated the effect of dietary L-Arg supplement on host immune responses during subsequent malaria infection using the Plasmodium yoelii 17XL BALB/c mouse model. We have shown that pretreatment of mice with L-Arg significantly decreased parasitemia and prolonged the survival time of mice after infection. L-Arg supplement led to significant increases in activated CD4+T-bet+IFN-?+ T cells and F4/80+CD36+ macrophages during early-stage infection, which were accompanied by enhanced synthesis of IFN-?, TNF-a and NO by spleen cells. Moreover, L-Arg-pretreated mice developed more splenic myeloid and plasmacytoid dendritic cells with up-regulated expression of MHC II, CD86 and TLR9. In comparison, L-Arg treatment did not change the number of regulatory T cells and the level of anti-inflammatory cytokine IL-10. Taken together, our results showed that L-Arg pretreatment could improve the protective immune response in experimental malaria infection in mice, which underlines potential importance of L-Arg supplement in malaria-endemic human populations.

引用

页码：412 / 420

页数：9

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