Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples

被引：39

作者：

Chan, Kwok Hung ^{[1
,2
]}

Yam, Wing Cheong ^{[1
,2
]}

Pang, Chiu Mei ^{[1
,2
]}

Chan, Kit Man ^{[1
,2
]}

Lam, Siu Yan ^{[1
,2
]}

Lo, Kam Fai ^{[1
,2
]}

Poon, Leo L. M. ^{[1
,2
]}

Peiris, J. S. Malik ^{[1
,2
,3
]}

机构：

[1] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[2] Queen Mary Hosp, Hong Kong, Hong Kong, Peoples R China

[3] HKU Pasteur Res Ctr, Pokfualm, Hong Kong, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 07期

关键词：

D O I：

10.1128/JCM.00315-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.

引用

页码：2195 / 2199

页数：5

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