Native mass spectrometry: a bridge between interactomics and structural biology

被引：604

作者：

Heck, Albert J. R. ^{[1
,2
]}

机构：

[1] Univ Utrecht, Bijvoet Ctr Biomol Res, Biomol Mass Spectrometry & Prote Grp, NL-3584 CA Utrecht, Netherlands

[2] Univ Utrecht, Utrecht Inst Pharmaceut Sci, NL-3584 CA Utrecht, Netherlands

来源：

NATURE METHODS | 2008年 / 5卷 / 11期

关键词：

D O I：

10.1038/nmeth.1265

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Native mass spectrometry is an emerging technology that allows the topological investigation of intact protein complexes with high sensitivity and a theoretically unrestricted mass range. This unique tool provides complementary information to established technologies in structural biology, and also provides a link to high-throughput interactomics studies, which do not generate information on exact protein complex-composition, structure or dynamics. Here I review the current state of native mass spectrometry technology and discuss several important biological applications. I also describe current experimental challenges in native mass spectrometry, encouraging readers to contribute to solutions.

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收藏

页码：927 / 933

页数：7

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