Potential of β-galactosidase-expressing Toxoplasma gondii for in situ localization and observation of rare stages of the parasite life cycle

被引：9

作者：

Dao, A

Soête, M

Sergent, V

Deslée, D

Fortier, B

Dubremetz, JF

机构：

[1] CHU Brabois Nancy, Serv Parasitol Mycol, F-54511 Vandoeuvre Les Nancy, France

[2] CNRS, FRE 2377, Inst Biol Lille, F-59021 Lille, France

来源：

PARASITOLOGY RESEARCH | 2002年 / 88卷 / 01期

关键词：

Escherichia Coli; Life Cycle; Microscopic Observation; Tissue Distribution; Electron Microscopic Observation;

D O I：

10.1007/s004360100499

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A cyst-forming strain of Toxoplasma gondii was transfected with the Escherichia coli LacZ gene and expressed beta-galactosidase constitutively. This strain has been used to localize and analyze the early stages of development and reactivation of T. gondii in mice. The chromogenic detection of the enzyme allows an easy detection of the parasites after light fixation and therefore allows a submacroscopic analysis of tissue distribution within the organism. Also, it allows further embedding and retrieval of rare stages for electron microscopic observation.

引用

页码：69 / 72

页数：4

共 5 条

[1] Successful reinfection of chronically infected mice by a different Toxoplasma gondii genotype [J].