Visualizing influenza virus infection in living mice

被引：93

作者：

Pan, Weiqi ^{[1
,2
]}

Dong, Zhenyuan ^{[1
]}

Li, Feng ^{[1
]}

Meng, Weixu ^{[1
,2
]}

Feng, Liqiang ^{[1
]}

Niu, Xuefeng ^{[2
]}

Li, Chufang ^{[2
]}

Luo, Qinfang ^{[2
]}

Li, Zhengfeng ^{[1
]}

Sun, Caijun ^{[1
]}

Chen, Ling ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, State Key Lab Resp Dis, Guangzhou 510530, Peoples R China

[2] Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou 510210, Peoples R China

来源：

NATURE COMMUNICATIONS | 2013年 / 4卷

关键词：

A VIRUS; REPLICATION; INTERFERON; ANTIBODIES; LUCIFERASE; DYNAMICS; VIRIONS; VECTOR; RNA;

D O I：

10.1038/ncomms3369

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Preventing and treating influenza virus infection remain a challenge because of incomplete understanding of the host-pathogen interactions, limited therapeutics and lack of a universal vaccine. So far, methods for monitoring the course of infection with influenza virus in real time in living animals are lacking. Here we report the visualization of influenza viral infection in living mice using an engineered replication-competent influenza A virus carrying luciferase reporter gene. After intranasal inoculation, bioluminescence can be detected in the chest and nasopharyngeal passage of living mice. The intensity of bioluminescence in the chest correlates with the dosage of infection and the viral load in the lung. Bioluminescence in the chest of infected mice diminishes on antiviral treatment. This work provides a novel approach that enables real-time study of influenza virus infection and effects of antiviral therapeutics in living animals.

引用

页数：8

共 19 条

[1] Luciferase Imaging of neurotropic viral infection in intact animals [J].

Cook, SH ;

Griffin, DE .

JOURNAL OF VIROLOGY, 2003, 77 (09) :5333-5338

[2]

Danielle M., 2012, MOL THER, V20, P692

[3] Rescue of influenza A virus from recombinant DNA [J].

Fodor, E ;

Devenish, L ;

Engelhardt, OG ;

Palese, P ;

Brownlee, GG ;

García-Sastre, A .

JOURNAL OF VIROLOGY, 1999, 73 (11) :9679-9682

[4] Selective incorporation of influenza virus RNA segments into virions [J].

Fujii, Y ;

Goto, H ;

Watanabe, T ;

Yoshida, T ;

Kawaoka, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (04) :2002-2007

[5] In Vivo Bioluminescent Imaging of Influenza A Virus Infection and Characterization of Novel Cross-Protective Monoclonal Antibodies [J].

Heaton, Nicholas S. ;

Leyva-Grado, Victor H. ;

Tan, Gene S. ;

Eggink, Dirk ;

Hai, Rong ;

Palese, Peter .

JOURNAL OF VIROLOGY, 2013, 87 (15) :8272-8281

[6] Genome packaging in influenza A virus [J].

Hutchinson, Edward C. ;

von Kirchbach, Johann C. ;

Gog, Julia R. ;

Digard, Paul .

JOURNAL OF GENERAL VIROLOGY, 2010, 91 :313-328

[7] Generation of Replication-Competent Recombinant Influenza A Viruses Carrying a Reporter Gene Harbored in the Neuraminidase Segment [J].

Li, Feng ;

Feng, Liqiang ;

Pan, Weiqi ;

Dong, Zhenyuan ;

Li, Chufang ;

Sun, Caijun ;

Chen, Ling .

JOURNAL OF VIROLOGY, 2010, 84 (22) :12075-12081

[8] Noninvasive bioluminescence imaging of herpes simplex virus type 1 infection and therapy in living mice [J].

Luker, GD ;

Bardill, JP ;

Prior, JL ;

Pica, CM ;

Piwnica-Worms, D ;

Leib, DA .

JOURNAL OF VIROLOGY, 2002, 76 (23) :12149-12161

[9] Bioluminescence imaging of vaccinia virus: Effects of interferon on viral replication and spread [J].

Luker, KE ;

Hutchens, M ;

Schultz, T ;

Pekosz, A ;

Luker, GD .

VIROLOGY, 2005, 341 (02) :284-300

[10] Analysis of in vivo dynamics of influenza virus infection in mice using a GFP reporter virus [J].

Manicassamy, Balaji ;

Manicassamy, Santhakumar ;

Belicha-Villanueva, Alan ;

Pisanelli, Giuseppe ;

Pulendran, Bali ;

Garcia-Sastre, Adolfo .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (25) :11531-11536

← 1 2 →