Detection of large pathogenic expansions in FRDA1, SCA10, and SCA12 genes using a simple fluorescent repeat-primed PCR assay

被引：41

作者：

Cagnoli, C

Michielotto, C

Matsuura, T

Ashizawa, T

Margolis, RL

Holmes, SE

Gellera, C

Migone, N

Brusco, A

机构：

[1] Univ Turin, Dipartimento Genet Biol & Biochim, I-10126 Turin, Italy

[2] Azienda Osped San giovanni Battista Torino, Turin, Italy

[3] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[4] Univ Texas, Med Branch, Dept Neurol, Galveston, TX 77550 USA

[5] Johns Hopkins Univ, Sch Med, Dept Psychiat, Baltimore, MD 21205 USA

[6] Ist Neurol Carlo Besta, Div Biochim & Genet, Milan, Italy

来源：

JOURNAL OF MOLECULAR DIAGNOSTICS | 2004年 / 6卷 / 02期

关键词：

D O I：

10.1016/S1525-1578(10)60496-5

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

At least 18 human genetic diseases are caused by expansion of short tandem repeats. Here we describe a successful application of a fluorescent PCR method for the detection of expanded repeats in FRDA1, SCA10, and SCA12 genes. Although this test cannot give a precise estimate of the size of the expansion, it is robust, reliable, and inexpensive, and can be used to screen large series of patients. it proved useful for confirming the presence of large expansions in the Friedreich ataxia gene following an ambiguous result of long-range PCR, as well as rapid pre-screening for large repeat expansions associated with Friedreich ataxia and SCA10 and the shorter repeat expansions associated with SCA12.

引用

页码：96 / 100

页数：5

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