Different ratios of sucrose/raffinose-induced membrane depolarizations in the mesophyll of species with symplasmic (Catharanthus roseus, Ocimum basilicum) or apoplasmic (Impatiens walleriana, Vicia faba) minor-vein configurations

被引:15
作者
vanBel, AJE
Hendriks, JHM
Boon, EJMC
Gamalei, YV
vandeMerwe, AP
机构
[1] DEPT PLANT ECOL & EVOLUT BIOL,TRANSPORT PHYSIOL RES GRP,3584 CA UTRECHT,NETHERLANDS
[2] VL KOMAROV BOT INST,DEPT ECOPHYSIOL,ST PETERSBURG 197376,RUSSIA
关键词
galactosyl oligosaccharides; membrane depolarization; phloem loading (apoplasmic; symplasmic); raffinose uptake; sucrose uptake; COLEUS-BLUMEI BENTH; COMMELINA-BENGHALENSIS; LEAF TISSUES; STACHYOSE SYNTHESIS; CUCURBITA-PEPO; LUCIFER YELLOW; SUCROSE UPTAKE; BETA-VULGARIS; PISUM-SATIVUM; SIEVE-TUBE;
D O I
10.1007/BF00196558
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In mesophyll cells of species with a symplasmic (Ocimum basilicum, Catharanthus roseus, Magnolia denudata) or an apoplasmic (Vicia faba, Impatiens walleriana, Bellis perennis) minor-vein configuration, membrane depolarizations in response to 20 or 200 mol . m(-3) raffinose and sucrose were measured. Ageing period and resting potential marginally affected the degree of depolarization. The symplasmic species showed similar depolarization responses to 20 and 200 mol . m(-3) sucrose or raffinose. In the apoplasmic species, depolarization increased statistically significantly from 20 to 200 mol . m(-3) sucrose, whereas the depolarization response to raffinose was equal at both concentrations. In the apoplasmic species, moreover, the depolarization response to raffinose was significantly weaker than to sucrose at all concentrations. A major difference between symplasmic and apoplasmic species seems to lie in the scantiness of raffinose carriers in the mesophyll plasma membrane of species with the apoplasmic mode of phloem loading.
引用
收藏
页码:185 / 192
页数:8
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