Microarrays of synthetic heparin oligosaccharides

被引：181

作者：

de Paz, JL ^{[1
]}

Noti, C ^{[1
]}

Seeberger, PH ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, ETH Zurich, Organ Chem Lab, CH-8093 Zurich, Switzerland

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 09期

关键词：

D O I：

10.1021/ja057584v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present the first preparation of microarrays containing synthetic heparin oligosaccharides in order to elucidate the heparin-protein interactions involved in a variety of biological processes. For this purpose, we have developed a novel linker strategy that is compatible with the protecting-group manipulations required for the synthesis of the highly sulfated oligosaccharides and can also be extended to an automated solid phase approach. Strategic placement of the orthogonally protected amine linker was key to the success of the array construction. These heparin chips allow for the high-throughput screening of oligosaccharides by using ∼picomoles of protein. The potential of the new method was demonstrated by probing the carbohydrate affinity of two heparin-binding growth factors, FGF-1 and FGF-2, that are implicated in the development and differentiation of several tumors. Copyright © 2006 American Chemical Society.

引用

页码：2766 / 2767

页数：2

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