A DNA extraction protocol from various tissues in woody species

被引：42

作者：

Cheng, FS

Brown, SK

Weeden, NF

机构：

来源：

HORTSCIENCE | 1997年 / 32卷 / 05期

关键词：

DNA isolation; fruit species; forest trees; spermine;

D O I：

10.21273/HORTSCI.32.5.921

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

A DNA extraction protocol was developed for tissues from woody species. DNA was extracted successfully from 11 species and five different types of tissues and was suitable for RAPD and restriction analysis. Spermine precipitation was used to further purify DNA. The protocol can be used for large-scale analysis and mini-preparations.

引用

页码：921 / 922

页数：2

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