Novel three-dimensional organoid model for evaluation of the interaction of uropathogenic Escherichia coli with terminally differentiated human urothelial cells

被引：68

作者：

Smith, YC ^{[1
]}

Grande, KK ^{[1
]}

Rasmussen, SB ^{[1
]}

O'Brien, AD ^{[1
]}

机构：

[1] Uniformed Serv Univ Hlth Sci, Dept Microbiol & Immunol, Bethesda, MD 20814 USA

来源：

INFECTION AND IMMUNITY | 2006年 / 74卷 / 01期

关键词：

D O I：

10.1128/IAI.74.1.750-757.2006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced alpha-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.

引用

页码：750 / 757

页数：8

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