Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases

被引：3000

作者：

Chen, Xi ^{[1
]}

Ba, Yi ^{[2
]}

Ma, Lijia ^{[3
,4
]}

Cai, Xing ^{[1
]}

Yin, Yuan ^{[1
]}

Wang, Kehui ^{[1
]}

Guo, Jigang ^{[1
]}

Zhang, Yujing ^{[1
]}

Chen, Jiangning ^{[1
]}

Guo, Xing ^{[1
]}

Li, Qibin ^{[3
,4
]}

Li, Xiaoying ^{[5
]}

Wang, Wenjing ^{[6
]}

Zhang, Yan ^{[1
]}

Wang, Jin ^{[1
]}

Jiang, Xueyuan ^{[1
]}

Xiang, Yang ^{[1
]}

Xu, Chen ^{[1
]}

Zheng, Pingping ^{[3
]}

Zhang, Juanbin ^{[3
]}

Li, Ruiqiang ^{[3
]}

Zhang, Hongjie ^{[1
]}

Shang, Xiaobin ^{[2
]}

Gong, Ting ^{[2
]}

Ning, Guang ^{[5
]}

Wang, Jun ^{[3
,4
]}

Zen, Ke ^{[1
]}

Zhang, Junfeng ^{[1
]}

Zhang, Chen-Yu ^{[1
]}

机构：

[1] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Jiangsu Diabet Ctr, Nanjing 210093, Jiangsu, Peoples R China

[2] Tianjin Med Univ Canc Inst & Hosp, Tianjin 300000, Peoples R China

[3] Beijing Genom Inst, Shenzhen 518083, Peoples R China

[4] Chinese Acad Sci, Beijing Inst Genom, Beijing 101300, Peoples R China

[5] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Endocrine & Metab Dis, Shanghai 200025, Peoples R China

[6] Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China

来源：

CELL RESEARCH | 2008年 / 18卷 / 10期

基金：

中国国家自然科学基金;

关键词：

serum-microRNA; expression profile; fingerprint; blood-based biomarker; Solexa; cancers; diabetes;

D O I：

10.1038/cr.2008.282

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.

引用

页码：997 / 1006

页数：10

共 20 条

[1] MicroRNA signatures in human cancers
Calin, George A.
Croce, Carlo M.
[J]. NATURE REVIEWS CANCER, 2006, 6 (11) : 857 - 866
[2] Real-time quantification of microRNAs by stem-loop RT-PCR
Chen, CF
Ridzon, DA
Broomer, AJ
Zhou, ZH
Lee, DH
Nguyen, JT
Barbisin, M
Xu, NL
Mahuvakar, VR
Andersen, MR
Lao, KQ
Livak, KJ
Guegler, KJ
[J]. NUCLEIC ACIDS RESEARCH, 2005, 33 (20) : e179.1 - e179.9
[3] Detection and characterization of placental MicroRNAs in maternal plasma
Chim, Stephen S. C.
Shing, Tristan K. F.
Hung, Emily C. W.
Leung, Tak-yeung
Lau, Tze-kin
Chiu, Rossa W. K.
Lo, Y. M. Dennis
[J]. CLINICAL CHEMISTRY, 2008, 54 (03) : 482 - 490
[4] Role of tumor markers in patients with solid cancers: A critical review
Duffy, Michael J.
[J]. EUROPEAN JOURNAL OF INTERNAL MEDICINE, 2007, 18 (03) : 175 - 184
[5] Clinical uses of tumor markers: A critical review
Duffy, MJ
[J]. CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES, 2001, 38 (03) : 225 - 262
[6] Characterization of amplifiable, circulating RNA in plasma and its potential as a tool for cancer diagnostics
El-Hefnawy, T
Raja, S
Kelly, L
Bigbee, WL
Kirkwood, JM
Luketich, JD
Godfrey, TE
[J]. CLINICAL CHEMISTRY, 2004, 50 (03) : 564 - 573
[7] Oncomirs - microRNAs with a role in cancer
Esquela-Kerscher, A
Slack, FJ
[J]. NATURE REVIEWS CANCER, 2006, 6 (04) : 259 - 269
[8] MicroRNA signatures of TRAIL resistance in human non-small cell lung cancer
Garofalo, M.
Quintavalle, C.
Di Leva, G.
Zanca, C.
Romano, G.
Taccioli, C.
Liu, C. G.
Croce, C. M.
Condorelli, G.
[J]. ONCOGENE, 2008, 27 (27) : 3845 - 3855
[9] Micro-RNA profiling in kidney and bladder cancers
Gottardo, Fedra
Liu, Chang Gong
Ferracin, Manuela
Calin, George A.
Fassan, Mattect
Bassi, Pierfrancesco
Sevignani, Cinzia
Byrne, Dolores
Negrini, Massimo
Pagano, Francesco
Gomella, Leonard G.
Croce, Carlo M.
Baffa, Raffaele
[J]. UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS, 2007, 25 (05) : 387 - 392
[10] Identification of microRNAs and other small regulatory RNAs using cDNA library sequencing
Hafner, Markus
Landgraf, Pablo
Ludwig, Janos
Rice, Amanda
Ojo, Tolulope
Lin, Carolina
Holoch, Daniel
Lim, Cindy
Tuschl, Thomas
[J]. METHODS, 2008, 44 (01) : 3 - 12

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