Enhancing the osteogenic efficacy of human bone marrow aspirate: concentrating osteoprogenitors using wave-assisted filtration

被引:30
作者
Dawson, Jonathan I. [1 ]
Smith, James O. [1 ,2 ]
Aarvold, Alexander [1 ,2 ]
Ridgway, Jonathan N. [3 ]
Curran, Steven J. [3 ]
Dunlop, Douglas G. [1 ,2 ]
Oreffo, Richard O. C. [1 ]
机构
[1] Univ Southampton, Sch Med, Bone & Joint Res Grp, York, N Yorkshire, England
[2] Univ Hosp Southampton NHS Fdn Trust, Dept Trauma & Orthoped, York, N Yorkshire, England
[3] Smith & Nephew Ltd, York, N Yorkshire, England
基金
英国工程与自然科学研究理事会;
关键词
bone marrow aspirate; bone marrow stem cells; bone regeneration; colony-forming unit-fibroblastoid; mesenchymal stem cells; osteoprogenitors; tissue engineering; MESENCHYMAL STEM-CELLS; TISSUE-ENGINEERING PRINCIPLES; MULTIPOTENTIAL STROMAL CELLS; HUMAN TRABECULAR BONE; PROGENITOR CELLS; IN-VITRO; DEMINERALIZED BONE; IMPACTED ALLOGRAFT; SEEDING DENSITY; DIFFERENTIATION;
D O I
10.1016/j.jcyt.2012.09.004
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
Background. Recent approaches have sought to harness the potential of stem cells to regenerate bone that is lost as a consequence of trauma or disease. Bone marrow aspirate (BMA) provides an autologous source of osteoprogenitors for such applications. However, previous studies indicated that the concentration of osteoprogenitors present in BMA is less than required for robust bone regeneration. We provide further evidence for the importance of BMA enrichment for skeletal tissue engineering strategies using a novel acoustic wave-facilitated filtration strategy to concentrate BMA for osteoprogenitors, clinically applicable for intraoperative orthopedic use. Methods. Femoral BMA from 15 patients of an elderly cohort was concentrated for the nucleated cell fraction against erythrocytes and excess plasma volume via size exclusion filtration facilitated by acoustic agitation. The effect of aspirate concentration was assessed by assays for colony formation, flow cytometry, multilineage differentiation and scaffold seeding efficiency. Results. BMA was filtered to achieve a mean 4.2-fold reduction in volume with a corresponding enrichment of viable and functional osteoprogenitors, indicated by flow cytometry and assays for colony formation. Enhanced osteogenic and chondrogenic differentiation was observed using concentrated aspirate and enhanced cell-seeding efficiency onto allogeneic bone graft as an effect of osteoprogenitor concentration relative specifically to the concentration of erythrocytes in the aspirate. Conclusions. These studies provide evidence for the importance of BMA nucleated cell concentration for both cell differentiation and cell seeding efficiency and demonstrate the potential of this approach for intraoperative application to enhance bone healing.
引用
收藏
页码:242 / 252
页数:11
相关论文
共 54 条
[1]
Aarvold A, 2011, REGEN MED, V6, P461, DOI [10.2217/rme.11.33, 10.2217/RME.11.33]
[2]
Anna V, 2009, J BONE JOINT SURG AM, V91, P1073
[3]
Treatment of chronic wounds with bone marrow-derived cells [J].
Badiavas, EV ;
Falanga, V .
ARCHIVES OF DERMATOLOGY, 2003, 139 (04) :510-516
[4]
Postnatal skeletal stem cells [J].
Bianco, Paolo ;
Kuznetsov, Sergei A. ;
Riminucci, Mara ;
Robey, Pamela Gehron .
ADULT STEM CELLS, 2006, 419 :117-148
[5]
Reconstruction of severe anterior maxillary defects using distraction osteogenesis, bone grafts, and implants [J].
Block, AS ;
Baughman, DG .
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2005, 63 (03) :291-297
[6]
The role and effectiveness of bone marrow in osseous regeneration [J].
Block, JE .
MEDICAL HYPOTHESES, 2005, 65 (04) :740-747
[7]
Biological and mechanical enhancement of impacted allograft seeded with human bone marrow stromal cells: potential clinical role in impaction bone grafting [J].
Bolland, Benjamin J. R. F. ;
Partridge, Kris ;
Tilley, Simon ;
New, Andrew M. R. ;
Dunlop, Douglas G. ;
Oreffo, Richard O. C. .
REGENERATIVE MEDICINE, 2006, 1 (04) :457-467
[8]
Bruder SP, 1999, CLIN ORTHOP RELAT R, pS68
[9]
Centeno Christopher J, 2006, Pain Physician, V9, P253
[10]
DEVELOPMENT OF AN OSTEOGENIC BONE-MARROW PREPARATION [J].
CONNOLLY, J ;
GUSE, R ;
LIPPIELLO, L ;
DEHNE, R .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1989, 71A (05) :684-691