Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development

被引：67

作者：

Huang, Quan-Sheng ^{[1
,2
,4
]}

Wang, Hai-Yun ^{[1
,3
]}

Gao, Peng ^{[1
,3
]}

Wang, Guo-Ying ^{[2
]}

Xia, Gui-Xian ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China

[2] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

[3] Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China

[4] Xinjiang Acad Agr Sci, Urumqi 830094, Xinjiang, Peoples R China

来源：

PLANT CELL REPORTS | 2008年 / 27卷 / 12期

关键词：

Gossypium; Calcium-dependent protein kinase; Cotton fiber development;

D O I：

10.1007/s00299-008-0603-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.

引用

收藏

页码：1869 / 1875

页数：7

相关论文

共 27 条

[1]

Phosphorylation of plant actin-depolymerising factor by calmodulin-like domain protein kinase [J].

Allwood, EG ;

Smertenko, AP ;

Hussey, PJ .

FEBS LETTERS, 2001, 499 (1-2) :97-100

[2]

Functional genomics of cell elongation in developing cotton fibers [J].

Arpat, AB ;

Waugh, M ;

Sullivan, JP ;

Gonzales, M ;

Frisch, D ;

Main, D ;

Wood, T ;

Leslie, A ;

Wing, RA ;

Wilkins, TA .

PLANT MOLECULAR BIOLOGY, 2004, 54 (06) :911-929

[3]

DEVELOPMENT OF THE COTTON FIBER [J].

BASRA, AS ;

MALIK, CP .

INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1984, 89 :65-113

[4]

EFFECTS OF PLANT-GROWTH SUBSTANCES ON IN-VITRO FIBER DEVELOPMENT FROM FERTILIZED COTTON OVULES [J].

BEASLEY, CA ;

TING, IP .

AMERICAN JOURNAL OF BOTANY, 1973, 60 (02) :130-139

[5]

Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family [J].

Cheng, SH ;

Willmann, MR ;

Chen, HC ;

Sheen, J .

PLANT PHYSIOLOGY, 2002, 129 (02) :469-485

[6]

Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis [J].

Dammann, C ;

Ichida, A ;

Hong, BM ;

Romanowsky, SM ;

Hrabak, EM ;

Harmon, AC ;

Pickard, BG ;

Harper, JF .

PLANT PHYSIOLOGY, 2003, 132 (04) :1840-1848

[7]

Identification of genes preferentially expressed in cotton fibers: A possible role of calcium signaling in cotton fiber elongation [J].

Gao, Peng ;

Zhao, Pi-Ming ;

Wang, Juan ;

Wang, Hai-Yun ;

Wu, Xiao-Min ;

Xia, Gui-Xian .

PLANT SCIENCE, 2007, 173 (01) :61-69

[8]

Characterization of eight new members of the calmodulin-like domain protein kinase gene family from Arabidopsis thaliana [J].

Hrabak, EM ;

Dickmann, LJ ;

Satterlee, JS ;

Sussman, MR .

PLANT MOLECULAR BIOLOGY, 1996, 31 (02) :405-412

[9]

RNA interference identifies a calcium-dependent protein kinase involved in Medicago truncatula root development [J].

Ivashuta, S ;

Liu, JY ;

Liu, JQ ;

Lohar, DP ;

Haridas, S ;

Bucciarelli, B ;

VandenBosch, KA ;

Vance, CP ;

Harrison, MJ ;

Gantt, JS .

PLANT CELL, 2005, 17 (11) :2911-2921

[10]

Isolation and analyses of genes preferentially expressed during early cotton fiber development by subtractive PCR and cDNA array [J].

Ji, SJ ;

Lu, YC ;

Feng, JX ;

Wei, G ;

Li, J ;

Shi, YH ;

Fu, Q ;

Liu, D ;

Luo, JC ;

Zhu, YX .

NUCLEIC ACIDS RESEARCH, 2003, 31 (10) :2534-2543