Characterization of leptospiral serovars by randomly amplified polymorphic DNA fingerprinting

被引：23

作者：

Ramadass, P

Meerarani, S

Venkatesha, MD

Senthilkumar, A

Nachimuthu, K

机构：

[1] Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Vet. and Anim. Sci. Univ., Chennai 600 007, Tamil Nadu

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1997年 / 47卷 / 02期

关键词：

D O I：

10.1099/00207713-47-2-575

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Randomly amplified polymorphic DNA (RAPD) fingerprinting of 14 laboratory strains of leptospiral serovars (serovars australis, autumnalis, ballum, bataviae, canicola. grippotyphosa, hardjoprajitno, hebdomadis, icterohaemorrhagiae, javanica, pomona, pyrogenes, panama, and tarassovi) was carried out by using a pair of primers. Each serovar had a unique and distinct fingerprint pattern. DNAs of other bacterial species, including Escherichia coli, Pasteurella multocida, Salmonella spp., Pseudomonas spp., and Klebsiella spp., did not show any amplification. RAPD fingerprinting was found to be a rapid and sensitive method for serovar identification when it was compared to DNA restriction enzyme analysis, which produced a larger number of bands that made it more difficult to compare serovars.

引用

页码：575 / 576

页数：2

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