Three-dimensional structure of mammalian casein kinase I: Molecular basis for phosphate recognition

被引:114
作者
Longenecker, KL [1 ]
Roach, PJ [1 ]
Hurley, TD [1 ]
机构
[1] INDIANA UNIV, SCH MED, DEPT BIOCHEM & MOLEC BIOL, INDIANAPOLIS, IN 46202 USA
关键词
anion binding; casein kinase I; crystallography; phosphorylation; protein kinase;
D O I
10.1006/jmbi.1996.0189
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The three-dimensional structure for the catalytic region of the mammalian protein kinase, casein kinase I delta (CKI delta), has been solved by X-ray crystallography to a resolution of 2.3 Angstrom. A truncation mutant of CKI delta lacking the C-terminal autoinhibitory region was expressed in Escherichia coli, purified, and crystallized. The structure was solved by molecular replacement using the crystal structure of the catalytic domain of a CKI homolog from Schizosaccharomyces pombe, Cki1. A tungstate derivative confirmed the initial molecular replacement solution and identified an anion binding site which may contribute to the unique substrate specificity of CKI. Like other protein kinases, the catalytic domain of CKI is composed of two lobes with a cleft between them for binding ATP. Comparison of the mammalian and yeast CKI structures suggests that a rotation of the N-terminal domain occurs upon ATP binding. This domain motion is similar, but not identical, to that observed in cAMP-dependent protein kinase upon binding ATP. Although Cki1 has many similarities to CKI delta over the catalytic domain, these two forms of CKI likely perform different functions in vivo. Relating the primary sequences of other CKI enzymes to the three-dimensional architecture of CKI delta reveals a catalytic face that is especially conserved among the subset of CKI family members associated with the regulation of DNA repair. (C) 1996 Academic Press Limited
引用
收藏
页码:618 / 631
页数:14
相关论文
共 56 条
  • [1] PHOSPHORYLATION OF THE PHOSPHATASE MODULATOR SUBUNIT (INHIBITOR-2) BY CASEIN KINASE-1 - IDENTIFICATION OF THE PHOSPHORYLATION SITES
    AGOSTINIS, P
    MARIN, O
    JAMES, P
    HENDRIX, P
    MERLEVEDE, W
    VANDENHEEDE, JR
    PINNA, LA
    [J]. FEBS LETTERS, 1992, 305 (02) : 121 - 124
  • [2] CRYSTAL-STRUCTURE OF HUMAN PROTEIN-TYROSINE-PHOSPHATASE 1B
    BARFORD, D
    FLINT, AJ
    TONKS, NK
    [J]. SCIENCE, 1994, 263 (5152) : 1397 - 1404
  • [3] CELL CYCLE-DEPENDENT LOCALIZATION OF CASEIN KINASE-I TO MITOTIC SPINDLES
    BROCKMAN, JL
    GROSS, SD
    SUSSMAN, MR
    ANDERSON, RA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) : 9454 - 9458
  • [4] ASSESSMENT OF PHASE ACCURACY BY CROSS VALIDATION - THE FREE R-VALUE - METHODS AND APPLICATIONS
    BRUNGER, AT
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1993, 49 : 24 - 36
  • [5] BRUNGER AT, 1992, XPLOR VERSION 3 1 SY
  • [6] CARMEL G, 1994, J BIOL CHEM, V269, P7304
  • [7] T-ANTIGEN KINASE INHIBITS SIMIAN-VIRUS 40 DNA-REPLICATION BY PHOSPHORYLATION OF INTACT T-ANTIGEN ON SERINE-120 AND SERINE-123
    CEGIELSKA, A
    MOAREFI, I
    FANNING, E
    VIRSHUP, DM
    [J]. JOURNAL OF VIROLOGY, 1994, 68 (01) : 269 - 275
  • [8] CHIJIWA T, 1989, J BIOL CHEM, V264, P4924
  • [9] DOMAIN MOVEMENTS IN PROTEIN-KINASES
    COX, S
    RADZIOANDZELM, E
    TAYLOR, SS
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1994, 4 (06) : 893 - 901
  • [10] CRYSTAL-STRUCTURE OF CYCLIN-DEPENDENT KINASE-2
    DEBONDT, HL
    ROSENBLATT, J
    JANCARIK, J
    JONES, HD
    MORGAN, DO
    KIM, SH
    [J]. NATURE, 1993, 363 (6430) : 595 - 602