A direct chemiluminescence method for the determination of nucleic acids using Ru(phen)32+-Ce(IV) system

A direct chemiluminescence method for the determination of nucleic acids has been developed based on the enhancement of nucleic acids on the chemiluminescence light emission of the reaction between Ru(phen)(3)(2+)(phen=1,10-phenanthroline) and Ce(IV). Under the optimum conditions, the calibration graphs are linear in the range of 5.0 x 10(-8)-5.0 x 10(-5) g/mL for calf thymus DNA, 8.0 x 10(-8)-5.0 x 10(-5) g/mL for fish sperm DNA and 1.0 x 10(-7)-3.0 x 10(-5) g/mL for yeast RNA, respectively. The limits of detection are 1.0 x 10(-5) g/mL for calf thymus DNA, 3.1 x 10(-8) g/mL for fish sperm DNA and 5.2 x 10(-8) g/mL for yeast RNA, respectively. The final procedure allows the successful determination of calf thymus DNA, fish sperm DNA and yeast RNA in six synthetic samples. This method is simple, rapid and specific.