Humanin inhibits cell death of serum-deprived PCI2h cells

被引：74

作者：

Kariya, S

Takahashi, N

Ooba, N

Kawahara, M

Nakayama, H

Ueno, S

机构：

[1] Nara Med Univ, Dept Neurol, Nara 6348522, Japan

[2] Nara Med Univ, Dept Pharmacol, Nara 6348522, Japan

来源：

NEUROREPORT | 2002年 / 13卷 / 06期

关键词：

Alzheimer's disease; amyloid-beta; cell death; humanin; neurodegenerative disease; PCI2; cell; serum deprivation;

D O I：

10.1097/00001756-200205070-00034

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Humanin (HN) and S14G HN (HNG) are recently discovered polypeptides that rescue cells from death induced by multiple different types of familial Alzheimer's disease genes and by amyloid-beta. However, the cytoprotective activity of these peptides against other cell death-inducing stimuli remains unclear. In this study, we demonstrated, using three different methods (MTS assay, caspase-3 assay, and detection of DNA fragmentation), that both HN and HNG protect PC12 cells from death elicited by serum deprivation. This implies the potential of the peptides to rescue cells from a broad spectrum, if not all, of cell death-inducing factors. Further investigations on HN may lead the possible application of this peptide as therapeutic agent for the treatment of other neurodegenerative diseases. NeuroReport 13:903-907 (C) 2002 Lippincott Williams Wilkins.

引用

页码：903 / 907

页数：5

共 25 条

[1] 5-(3-CARBOXYMETHOXYPHENYL)-2-(4,5-DIMETHYLTHIAZOLYL)-3-(4-SULFOPHENYL)TETRAZOLIUM, INNER SALT (MTS) AND RELATED ANALOGS OF 3-(4,5-DIMETHYLTHIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) REDUCING TO PURPLE WATER-SOLUBLE FORMAZANS AS CELL-VIABILITY INDICATORS [J].