Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506

被引：170

作者：

Carbajo-Lozoya, Javier ^{[1
]}

Mueller, Marcel A. ^{[2
]}

Kallies, Stephan ^{[2
]}

Thiel, Volker ^{[3
,4
]}

Drosten, Christian ^{[2
]}

von Brunn, Albrecht ^{[1
]}

机构：

[1] Univ Munich, Max Von Pettenkofer Inst, D-80336 Munich, Germany

[2] Univ Bonn, Med Ctr, Inst Virol, D-53105 Bonn, Germany

[3] Kantonsspital St Gallen, Inst Immunobiol, St Gallen, Switzerland

[4] Univ Zurich, Fac Vet, CH-8006 Zurich, Switzerland

来源：

VIRUS RESEARCH | 2012年 / 165卷 / 01期

关键词：

SARS-CoV; HCoV-NL63; HCoV-229E; FK506; Tacrolimus; Immunophilins; FKBP1A (FKBP12); FKBP1B (FKBP12.6); Inhibition of viral replication; ACUTE RESPIRATORY SYNDROME; C VIRUS-REPLICATION; CYCLOSPORINE-A; INFECTION; IDENTIFICATION; CYCLOPHILIN; CELLS; IMMUNOPHILINS; CHLOROQUINE; THERAPIES;

D O I：

10.1016/j.virusres.2012.02.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent research has shown that Coronavirus (Coy) replication depends on active immunophilin pathways. Here we demonstrate that the drug FK506 (Tacrolimus) inhibited strongly the growth of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E at low, non-cytotoxic concentrations in cell culture. As shown by plaque titration, qPCR, Luciferase- and green fluorescent protein (GFP) reporter gene expression, replication was diminished by several orders of magnitude. Knockdown of the cellular FK506-binding proteins FKBP1A and FKBP1B in CaCo2 cells prevented replication of HCoV-NL63, suggesting the requirement of these members of the immunophilin family for virus growth. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：112 / 117

页数：6

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